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KMID : 0378019690120120035
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New Medical Journal
1969 Volume.12 No. 12 p.35 ~ p.38
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The Identification of Cholera Vibrio Isolated from the Cholera Epidemic in Korea on September 1969
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張鶴來/Jahng, Hack Nae
崔大卿/李楨伯/柳駿/Choi, Tai Kyung/Lee, Jung Baik/Le, Joon
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Abstract
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As mentioned on the previous report about the studies on cholera, trypsin test, hexamine test and phage typing with cholera phage type. IV were added there-after, to confirm the identity of cholera vibrio. From the above test results, no contradictory data were obtained to the conclusion of the first report in which the etiological agent was identified as V. cholerae biotype El tor, serotype ogawa.
Hemolysis test and polymyxin B sensitivity test were performed in more detail. On hemolysis test, when Feeley´s method, instead of Greig´s method, was carried out with 375 strains including two classic´ cholera controls and three El for cholera controls, about 55 strains showed hemolysis.
Besides these, 2 classic controls showed hemolysis positive. From these data, author suggested the hemolysis test as a poor differentiating method inbetween True and El for cholera.
On polymyxin B sensitivity test, author inoculated the strains under test and controls on the media containing polymyxin B serially diluted. Two True cholera strains were inhibited on 4´ gamma, whereas two El tor controls and strains under test were resisted even on 100 gamma. It was found the inhibitory range of polymixin B. (between true and El for cholera was) broad enough. And polymyxin B sensitivity test was recommandable as a relatively preferable test method on this occasion.
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